Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Following DNA damage, human cells activate essential repair pathways to restore the integrity of their genome. Here, we describe the method of indirect immunofluorescence as a means to detect DNA repair proteins, analyze their spatial and temporal recruitment, and help interrogate protein-protein in...

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Bibliographic Details
Main Author: de la Pen̳a Avalos, Ba̹rbara
Format: Electronic Video
Language:English
Published: Cambridge, MA MyJoVE Corp 2016
Series:Biology
Subjects:
Online Access:CONNECT