Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm

Imprinting is a phenomenon in plant and mammal reproduction. DNA methylation plays an important role in mechanisms of imprinting. Isolating endosperm and determining methylation status of imprinted genes in Arabidopsis can be difficult. In this protocol, we describe how to isolate endosperm and dete...

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Bibliographic Details
Main Author: Rea, Matthew
Format: Electronic Video
Language:English
Published: Cambridge, MA MyJoVE Corp 2016
Series:Biology
Subjects:
Online Access:CONNECT