DNA Electroporation, Isolation and Imaging of Myofibers
This protocol utilizes electroporation to introduce and express fluorescently labeled proteins in mouse muscle fibers. Following recovery after electroporation, fibers are isolated. Individual fibers are then imaged using high resolution confocal microscopy to visualize muscle structure.
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| Main Author: | |
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| Format: | Electronic Video |
| Language: | English |
| Published: |
Cambridge, MA
MyJoVE Corp
2016
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| Series: | Biology
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| Subjects: | |
| Online Access: | CONNECT |
MARC
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| 520 | |a This protocol utilizes electroporation to introduce and express fluorescently labeled proteins in mouse muscle fibers. Following recovery after electroporation, fibers are isolated. Individual fibers are then imaged using high resolution confocal microscopy to visualize muscle structure. | ||
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