DNA Electroporation, Isolation and Imaging of Myofibers

This protocol utilizes electroporation to introduce and express fluorescently labeled proteins in mouse muscle fibers. Following recovery after electroporation, fibers are isolated. Individual fibers are then imaged using high resolution confocal microscopy to visualize muscle structure.

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Bibliographic Details
Main Author: Demonbreun, Alexis R.
Format: Electronic Video
Language:English
Published: Cambridge, MA MyJoVE Corp 2016
Series:Biology
Subjects:
Online Access:CONNECT