An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
We provide here an efficient and reliable protocol for immunostaining, Fluorescence in situ Hybridization, DNA staining followed by quantitative, high-resolution imaging in whole-mount Arabidopsis thaliana ovules. This method was successfully used to analyze chromatin modifications and nuclear archi...
Saved in:
| Main Author: | |
|---|---|
| Format: | Electronic Video |
| Language: | English |
| Published: |
Cambridge, MA
MyJoVE Corp
2016
|
| Series: | Biology
|
| Subjects: | |
| Online Access: | CONNECT |
