Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
SILAC immunoprecipitation experiments represent a powerful means for discovering novel protein:protein interactions. By allowing the accurate relative quantification of protein abundance in both control and test samples, true interactions may be easily distinguished from experimental contaminants, a...
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Main Author: | |
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Format: | Electronic Video |
Language: | English |
Published: |
Cambridge, MA
MyJoVE Corp
2016
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Series: | Biology
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Subjects: | |
Online Access: | CONNECT |