Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.
This procedure describes a method for the isolation and culture of the murine organ of Corti with or without the spiral limbus and spiral ganglion neurons. We also demonstrate a method for the expression of an exogenous reporter gene in the organ of Corti explant by electroporation.
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| Main Author: | |
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| Format: | Electronic Video |
| Language: | English |
| Published: |
Cambridge, MA
MyJoVE Corp
2016
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| Series: | Biology
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| Subjects: | |
| Online Access: | CONNECT |
