In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
We describe a method to conditionally knockdown the expression of a target protein during adult zebrafish fin regeneration. This technique involves micro-injecting and electroporating antisense oligonucleotide morpholinos into fin tissue, which allows testing the protein's role in various stage...
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Main Author: | |
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Format: | Electronic Video |
Language: | English |
Published: |
Cambridge, MA
MyJoVE Corp
2016
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Series: | Biology
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Subjects: | |
Online Access: | CONNECT |