Identification of protein complexes with quantitative proteomics in S. cerevisiae
Here we describe a new quantitative proteomics technique for identifying protein complexes in Saccharomyces cerevisiae. In this study, we have used the SILAC method together with affinity purification followed by tandem mass spectrometry to identify with high specificity the binding partners of an E...
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| Main Author: | |
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| Format: | Electronic Video |
| Language: | English |
| Published: |
Cambridge, MA
MyJoVE Corp
2016
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| Series: | Biology
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| Subjects: | |
| Online Access: | CONNECT |
