Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice

Two-photon imaging has uncovered lymphocyte motility and cellular interactions within the lymph node under basal conditions and durring an immune response 1. Here, we demonstrate adoptive transfer of T cells, isolation of lymph nodes, and imaging motility of CD4+ T cells in the explanted lymph nod...

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Bibliographic Details
Main Author: Matheu, Melanie P.
Format: Electronic Video
Language:English
Published: Cambridge, MA MyJoVE Corp 2016
Series:Biology
Subjects:
Online Access:CONNECT