RNAi Trigger Delivery into Anopheles gambiae Pupae
RNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during...
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Main Author: | |
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Format: | Electronic Video |
Language: | English |
Published: |
Cambridge, MA
MyJoVE Corp
2016
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Series: | Biology
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Subjects: | |
Online Access: | CONNECT |
Summary: | RNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during pupal development. |
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Item Description: | JoVE Journal: Biology |
Physical Description: | 1 online resource (598 secs.) |
Audience: | For undergraduate, graduate, and professional students |